PVX is mostly latent infection, with no obvious symptoms, sometimes slight mottle, and in severe cases, it will cause symptoms such as wrinkled leaf mosaic . . . .
Scope of application
: RT 10339050
: Agitest Plant Virus Rapid Test - Potato Virus X
: Seed potato growers, importers and exporters, quarantine screening
: Less than 15 minutes
: 50 test strips per box
Potato (Solanum tuberosum L.) is a plant of Solanaceae family, its underground tubers are edible and it is one of the main food crops in the world. Propagation of potato is mainly asexual by seed potato. In Taiwan, seed potatoes are either mainly imported, or from breeding centers and supply system, or by potato farmers. However, the potato seeds could be highly variable, and pathogens are easily transmitted through seed potato to healthy plants, resulting in multi-infections of virus diseases, and in turn causing big loss in potato production. Therefore, the quality of seed potato is crucial to the quality and yield of consumable potato.
Virus transmission can not only trough seed potato, but also mechanical contact, e.g. tools and insect bites. The common symptoms of infection mainly appear on leaves, usually mosaic symptoms. The leaves are yellow-green uneven phenomenon, and occasionally gangrenous streaks or water dipping spots, the surface of the affected leaves is uneven, shrunken or deformed, the color of the new leaves becomes pale yellow, the plants are dwarfed, and the stems are dwarfed or clustered. The main types of potato viruses are Potato virus Y (PVY), Potato virus S (PVS), Potato virus X (PVX), Potato leaf roll virus (PLRV). The detection method in lab adopts ELISA detection and RT-PCR detection.
Potato virus X (PVX) is mostly latent infection, with no obvious symptoms, sometimes slight mottle on leaves. In some rare severe cases, PVX also causes leaf mosaic, dwarf plant, and small new leaves. Some PVX strains infect specific potato breeds can cause necrosis on the top of plant, plant death, and tuber necrosis. Transmission of PVX can be through infected seed potatoes and mechanical contacts. Sap contains virus can be transmitted by wind, injuries by animals, root-root contact between plants, cutting implements, or biting insects (such as grasshoppers, locusts).
Lateral flow rapid test can reach a similar sensitivity and specificity to ELISA, but surpasses ELISA in the simplicity of operation and saving of time on virus detection. Considering of its advantages of no need for equipment and low cost, making rapid test suitable for on-site and real-time virus testing and monitor in potato farms, and thus being a good tool for potato farmers and quarantine agencies to do virus control. Agitest Potato Virus X Rapid Test Strip is able to detect trace amount of PVX in potato leaves within 15 minutes. No equipment is required for conducting the test, and results can be interpreted visually. Strips can be stored and transported at room temperature environment.
Example of test operation / Potato virus Y rapid test strip