PVA-infected leaves show mild mosaic, dark green uneven, slightly rippling leaves, and some varieties also have gangrenous spots . . .
Scope of application
: RT 10359050
: Agitest Plant Virus Rapid Test - Potato Virus A
: Seed potato growers, importers and exporters, quarantine screening
: Less than 15 minutes
: 50 test strips per box
Potato (Solanum tuberosum L.) is a plant of Solanaceae family, its underground tubers are edible and it is one of the main food crops in the world. Propagation of potato is mainly asexual by seed potato. In Taiwan, seed potatoes are either mainly imported, or from breeding centers and supply system, or by potato farmers. However, the potato seeds could be highly variable, and pathogens are easily transmitted through seed potato to healthy plants, resulting in multi-infections of virus diseases, and in turn causing big loss in potato production. Therefore, the quality of seed potato is crucial to the quality and yield of consumable potato.
Virus transmission can not only trough seed potato, but also mechanical contact, e.g. tools and insect bites. The common symptoms of infection mainly appear on leaves, usually mosaic symptoms. The leaves are yellow-green uneven phenomenon, and occasionally gangrenous streaks or water dipping spots, the surface of the affected leaves is uneven, shrunken or deformed, the color of the new leaves becomes pale yellow, the plants are dwarfed, and the stems are dwarfed or clustered. The main types of potato viruses are Potato virus Y (PVY), Potato virus S (PVS), Potato virus X (PVX), Potato leaf roll virus (PLRV). The detection method in lab adopts ELISA detection and RT-PCR detection.
Potato virus A (PVA) , the infected leaves show mild mosaic, uneven color, slight rippling leaves. Some varieties also have gangrenous spots. Co-infection of PVA with PVX or PVY results in wrinkled leaves, rippling leaves, and signs of gangrene. Transmission of PVA can be through contaminated cutting tools, or aphids in a non-persistent manner.
Lateral flow rapid test can reach a similar sensitivity and specificity to ELISA, but surpasses ELISA in the simplicity of operation and saving of time on virus detection. Considering of its advantages of no need for equipment and low cost, making rapid test suitable for on-site and real-time virus testing and monitor in potato farms, and thus being a good tool for potato farmers and quarantine agencies to do virus control. Agitest Potato Virus A Rapid Test Strip is able to detect trace amount of PVA in potato leaves within 15 minutes. No equipment is required for conducting the test, and results can be interpreted visually. Strips can be stored and transported at room temperature environment.
Example of testing operation / Potato virus Y rapid test strip